Proposal of Model for Evaluation of Viral Kinetics of African/Asian/Brazilian—Zika virus Strains (Step Growth Curve) in Trophoblastic Cell Lines

The Zika virus (ZIKV) epidemic brought new discoveries regarding arboviruses, especially flaviviruses, as ZIKV was described sexually and vertically transmitted. latter shows severe consequences for the embryo/fetus, such congenital microcephaly deficiency of neural system, currently known Congenital Syndrome (CZS). To better understand dynamics in trophoblastic cells present first trimester pregnancy (BeWo, HTR-8, control cell HuH-7), an experiment viral kinetics performed African MR766 low passage Asian-Brazilian IEC lineages. results were independently demonstrated that three placental lines are permissive susceptible to ZIKV. We noticed cytopathic effects typical vitro infection BeWo HTR-8. Regarding kinetics, MR766lp showed peaks loads 24 48 hpi all types tested, well marked death after peak production. On other hand, HTR-8 lineage inoculated with ZIKV-IEC exhibited increased production 144 hpi, a between 96 hpi. Furthermore, had variations Considering results, hypothesis maternal fetal transmission is probably way mother embryo/fetus maintained.